anti-Human TANK-Binding Kinase 1 antibody for Immunohistochemistry

Recommended TANK-Binding Kinase 1 Antibody (supplied by: Log in to see )

Antigen
TANK-Binding Kinase 1 (TBK1) Antibodies
  • nak
  • t2k
  • TBK1
  • wu:fk70c05
  • zgc:136548
  • NAK
  • T2K
  • 1200008B05Rik
  • AI462036
  • AW048562
  • TANK binding kinase 1 L homeolog
  • TANK binding kinase 1
  • TANK-binding kinase 1
  • tbk1.L
  • TBK1
  • tbk1
  • Tbk1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
133
82
46
26
24
9
9
8
7
5
4
3
2
1
1
1
1
1
Host
Mouse
106
34
13
Clonality
Monoclonal
Conjugate
Un-conjugated
10
10
9
7
5
5
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
134
71
61
30
23
18
9
6
3
2
1
1
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Catalog No. ABIN252648
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.96781 ABIN6271546 ELISA IHC WB Rabbit IgG pSer172 Log in to see Polyclonal 0
15.811332 ABIN3016268 IHC WB Rabbit Log in to see Polyclonal 0
12.811332 ABIN3187188 ELISA IHC Rabbit IgG Tyr699 Log in to see Polyclonal 0
10 ABIN1871368 ICC IHC WB Rabbit IgG AA 9-310 Log in to see Polyclonal 0
10 ABIN2913758 IF/ICC IHC IP WB Rabbit AA 9-310 Log in to see Polyclonal 0
9.96781 ABIN768997 IHC IHC (p) WB Rabbit IgG AA 74-123 Log in to see Polyclonal 0
9.96781 ABIN768998 IHC IHC (p) WB Rabbit IgG AA 539-588 Log in to see Polyclonal 0
9.96781 ABIN213888 IHC IHC (p) WB Mouse IgG1, kappa AA 563-577 Log in to see 108A429  0
9.96781 ABIN6265480 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
9.96781 ABIN3029094 IHC ELISA WB Rabbit Ig Fraction AA 150-181 Log in to see Polyclonal 0
7 ABIN1875027 IHC WB Rabbit IgG Log in to see Polyclonal 0
6.9678097 ABIN2625929 ELISA IHC IHC (p) WB Goat AA 514-527 Log in to see Polyclonal 0
1 ABIN2625928 IHC IHC (p) WB Rabbit IgG AA 577-590 Log in to see Polyclonal 0
1 ABIN1450668 IHC ELISA Rabbit IgG AA 138-187 Log in to see Polyclonal 0
1 ABIN2878398 ELISA IHC IHC (p) Rabbit IgG AA 138-187 Log in to see Polyclonal 0
1 ABIN1952776 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser172 Log in to see Polyclonal 0
1 ABIN1954947 IHC ELISA WB Biotin Rabbit IgG Ser172 Log in to see Polyclonal 0
1 ABIN1953648 IHC ELISA WB APC Rabbit IgG Ser172 Log in to see Polyclonal 0
1 ABIN1958421 IHC ELISA WB PE Rabbit IgG Ser172 Log in to see Polyclonal 0
1 ABIN1956094 IHC ELISA WB FITC Rabbit IgG Ser172 Log in to see Polyclonal 0

General

Antigen TANK-Binding Kinase 1 (TBK1) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(133), (82), (46), (26), (24), (9), (9), (8), (7), (5), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(106), (34), (13)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(10), (10), (9), (7), (5), (5), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(134), (71), (61), (30), (23), (18), (9), (6), (3), (2), (1), (1)
Pubmed 22 references available
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Product details

Target Details Application Details Handling References Images
Purification Protein G purified
Immunogen A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
Clone 108A429
Isotype IgG1 kappa

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name TBK1 (TBK1 Antibody Abstract)
Background Gene Symbol: TBK1
Molecular Weight Theoretical MW: 84 kDa
Gene ID 29110
UniProt Q9UHD2
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western Blot 1-2 μg/mL, Simple Western 10 μg/mL, Immunohistochemistry 1:20-1:1000, Immunocytochemistry/Immunofluorescence 1:20, Immunohistochemistry-Paraffin 1:20-1:1000In Western Blot this antibody detects a band at 80 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

Gulen, Koch, Haag, Schuler, Apetoh, Villunger, Radtke, Ablasser: "Signalling strength determines proapoptotic functions of STING." in: Nature communications, Vol. 8, Issue 1, pp. 427, 2018 Method employed by authors: Western Blotting (WB)

Kwon, Park, Sesaki, Kang: "Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) suppresses STING-mediated DNA sensing pathway through inducing mitochondrial fission." in: Biochemical and biophysical research communications, Vol. 493, Issue 1, pp. 737-743, 2017 Method employed by authors: Western Blotting (WB)

Slowicka, Vereecke, Mc Guire, Sze, Maelfait, Kolpe, Saelens, Beyaert, van Loo: "Optineurin deficiency in mice is associated with increased sensitivity to Salmonella but does not affect proinflammatory NF-κB signaling." in: European journal of immunology, Vol. 46, Issue 4, pp. 971-80, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Mukawera, Chartier, Williams, Pagano, Lapointe, Grandvaux: "Redox-modulating agents target NOX2-dependent IKKε oncogenic kinase expression and proliferation in human breast cancer cell lines." in: Redox biology, Vol. 6, pp. 9-18, 2015 (Sample species: Human). Further details: Western Blotting

Yu, Croxen, Marchiando, Ferreira, Cadwell, Foster, Finlay: "Autophagy facilitates Salmonella replication in HeLa cells." in: mBio, Vol. 5, Issue 2, pp. e00865-14, 2014 (Sample species: Human). Further details: Western Blotting

Conlon, Burdette, Sharma, Bhat, Thompson, Jiang, Rathinam, Monks, Jin, Xiao, Vogel, Vance, Fitzgerald: "Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 10, pp. 5216-25, 2013

Tamassia, Bazzoni, Le Moigne, Calzetti, Masala, Grisendi, Bussmeyer, Scutera, De Gironcoli, Costantini, Musso, Cassatella: "IFN-β expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 3, pp. 1500-9, 2012 Further details: Western Blotting

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Ogasawara, Sasaki, Itoh, Tokudome, Kondo, Ito, Tanida, Kamiya, Kataoka, Joh, Kasugai: "Rebamipide suppresses TLR-TBK1 signaling pathway resulting in regulating IRF3/7 and IFN-α/β reduction." in: Journal of clinical biochemistry and nutrition, Vol. 48, Issue 2, pp. 154-60, 2011 (Sample species: Human). Further details: Western Blotting

Berthet, Damien, Hamzeh-Cognasse, Pozzetto, Garraud, Cognasse: "Toll-like receptor 4 signal transduction in platelets: novel pathways." in: British journal of haematology, Vol. 151, Issue 1, pp. 89-92, 2010 (Sample species: Human). Further details: Western Blotting

Marchlik, Thakker, Carlson, Jiang, Ryan, Marusic, Goutagny, Kuang, Askew, Roberts, Benoit, Zhou, Ling, Pfeifer, Stedman, Fitzgerald, Lin, Hall: "Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality." in: Journal of leukocyte biology, Vol. 88, Issue 6, pp. 1171-80, 2010 (Sample species: Mouse (Murine)). Further details: Western Blotting

Joung, Park, Rani, Takeuchi, Akira, Lee: "Akt contributes to activation of the TRIF-dependent signaling pathways of TLRs by interacting with TANK-binding kinase 1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 1, pp. 499-507, 2010 Further details: Western Blotting

Radtke, ORiordan: "Homeostatic maintenance of pathogen-containing vacuoles requires TBK1-dependent regulation of aquaporin-1." in: Cellular microbiology, Vol. 10, Issue 11, pp. 2197-207, 2008

McCoy, Carpenter, Pålsson-McDermott, Gearing, ONeill: "Glucocorticoids inhibit IRF3 phosphorylation in response to Toll-like receptor-3 and -4 by targeting TBK1 activation." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14277-85, 2008 (Sample species: Human). Further details: Western Blotting

Tamassia, Le Moigne, Rossato, Donini, McCartney, Calzetti, Colonna, Bazzoni, Cassatella: "Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 9, pp. 6563-73, 2008 (Sample species: Human). Further details: Western Blotting

Merimi, Klener, Szynal, Cleuter, Kerkhofs, Burny, Martiat, Van den Broeke: "Suppression of viral gene expression in bovine leukemia virus-associated B-cell malignancy: interplay of epigenetic modifications leading to chromatin with a repressive histone code." in: Journal of virology, Vol. 81, Issue 11, pp. 5929-39, 2007

Aksoy, Albarani, Nguyen, Laes, Ruelle, De Wit, Willems, Goldman, Goriely: "Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells." in: Blood, Vol. 109, Issue 7, pp. 2887-93, 2007 (Sample species: Human). Further details: Western Blotting

Péant, Diallo, Lessard, Delvoye, Le Page, Saad, Mes-Masson: "Regulation of IkappaB kinase epsilon expression by the androgen receptor and the nuclear factor-kappaB transcription factor in prostate cancer." in: Molecular cancer research : MCR, Vol. 5, Issue 1, pp. 87-94, 2007 (Sample species: Human). Further details: Western Blotting

Tamassia, Le Moigne, Calzetti, Donini, Gasperini, Ear, Cloutier, Martinez, Fabbri, Locati, Mantovani, McDonald, Cassatella: "The MyD88-independent pathway is not mobilized in human neutrophils stimulated via TLR4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 7344-56, 2007 (Sample species: Human).

Sankar, Chan, Romanow, Li, Bates: "IKK-i signals through IRF3 and NFkappaB to mediate the production of inflammatory cytokines." in: Cellular signalling, Vol. 18, Issue 7, pp. 982-93, 2006 (Sample species: Human).

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Western blot analysis of TBK1 using ABIN252648 at 2 ug/ml dilution against 10 ug/lane...
Western Blotting (WB) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Western blot analysis of TBK1 in A) human Daudi cell lysate and B) mouse RAW cell lys...
Western Blotting (WB) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Western Blot: TBK1 Antibody (108A429) [ABIN2526485] - Analysis using Azide and BSA Fr...
Western Blotting (WB) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Western Blot: TBK1 Antibody (108A429) [ABIN2526485] - Simple Western lane view shows ...
Western Blotting (WB) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Western Blot: TBK1 Antibody (108A429) [ABIN2526485] - Analysis using Azide and BSA Fr...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [ABIN2526485] - Analysis using...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [ABIN2526485] - Human testis (...
Simple Western (SimWes) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Simple Western: TBK1 Antibody (108A429) [ABIN2526485] - Analysis using Azide and BSA ...
Immunohistochemistry (IHC) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Immunohistochemistry: TBK1 Antibody (108A429) [ABIN2526485] - Antibody was tested in ...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-TANK-Binding Kinase 1 (TBK1) antibody (ABIN252648) Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) - HeLa cells were fi...