anti-Human TLR3 antibody for Immunofluorescence

Recommended TLR3 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 3 (TLR3) Antibodies
  • CD283
  • IIAE2
  • AI957183
  • zgc:123006
  • toll like receptor 3
  • toll-like receptor 3
  • TLR3
  • Tlr3
  • tlr3
Reactivity
Human
276
207
64
45
5
4
3
2
2
2
1
1
1
1
1
Host
Mouse
198
119
39
3
3
Clonality
Monoclonal
Conjugate
This TLR3 antibody is conjugated to Biotin
33
30
24
16
10
8
7
6
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
235
139
103
89
64
42
40
34
14
13
12
7
5
4
1
1
Options
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Catalog No. ABIN2191974
$ 405.93
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.755764 ABIN2191973 FACS IF IHC (p) FITC Mouse IgG1 Log in to see TLR3-7 6
21.755764 ABIN2191972 FACS IF IHC (p) Mouse IgG1 Log in to see TLR3-7 6
20.30825 ABIN615251 EIA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
17.30825 ABIN615847 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
10 ABIN604951 ICC IF IHC IHC (p) WB Rabbit AA 514-657 Log in to see Polyclonal 0
7 ABIN2663312 ICC FACS IF PE Mouse IgG2a kappa Log in to see TLR-104 8
7 ABIN1740109 ICC IF IHC (p) WB Rabbit IgG AA 514-657 Log in to see Polyclonal 0
7 ABIN187555 FACS IF IHC (p) IP WB Mouse IgG1 Log in to see 40C1285-6 0
4 ABIN265139 IF WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN5555333 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5555334 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1106214 Func IHC (fro) IF IHC (p) IP WB FITC Mouse IgG1 Log in to see TLR3-7 4
1 ABIN1106212 Func IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 Log in to see TLR3-7 5
1 ABIN1106213 Func IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 Log in to see TLR3-7 4
1 ABIN2376253 IC IF IHC WB Rabbit AA 514-657 Log in to see Polyclonal 0
1 ABIN2376228 IC IF ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1175322 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2467253 ICC IF IHC WB Rabbit AA 514-657 Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 3 (TLR3) Antibodies
Reactivity Human
(276), (207), (64), (45), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(198), (119), (39), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TLR3 antibody is conjugated to Biotin
(33), (30), (24), (16), (10), (8), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(235), (139), (103), (89), (64), (42), (40), (34), (14), (13), (12), (7), (5), (4), (1), (1)
Pubmed 6 references available
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Product Details anti-TLR3 Antibody

Target Details TLR3 Application Details Handling References for anti-TLR3 antibody (ABIN2191974) Images
Cross-Reactivity (Details) Cross reactivity: 4 Canine TLR3 : Yes, Mouse TLR3 : Yes
Sterility 0.2 μm filtered
Clone TLR3-7
Isotype IgG1
Plasmids, Primers & others

Target Details TLR3

Product Details anti-TLR3 Antibody Application Details Handling References for anti-TLR3 antibody (ABIN2191974) Images back to top
Antigen
Alternative Name Toll-Like Receptor 3 (TLR3 Antibody Abstract)
Background The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB- dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses. Aliases CD283 Immunogen Human Flag-tagged TLR3 stably expressed by Ba/F3 cells
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR3 Antibody Target Details TLR3 Handling References for anti-TLR3 antibody (ABIN2191974) Images back to top
Application Notes For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells control Negative HEK293 control
Restrictions For Research Use only

Handling

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details References for anti-TLR3 antibody (ABIN2191974) Images back to top
Buffer PBS, containing 1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-TLR3 antibody (ABIN2191974)

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details Handling Images back to top
Product cited in:

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Schreiner, Voss, Wischhusen, Dombrowski, Steinle, Lochmüller, Dalakas, Melms, Wiendl: "Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 1, pp. 118-20, 2006

Jorgenson, Young, Lesmeister, Lyddon, Misfeldt: "Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA." in: Human immunology, Vol. 66, Issue 5, pp. 469-82, 2005

Oshiumi, Matsumoto, Funami, Akazawa, Seya: "TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction." in: Nature immunology, Vol. 4, Issue 2, pp. 161-7, 2003

Matsumoto, Funami, Tanabe, Oshiumi, Shingai, Seto, Yamamoto, Seya: "Subcellular localization of Toll-like receptor 3 in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 3154-62, 2003

Matsumoto, Kikkawa, Kohase, Miyake, Seya: "Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling." in: Biochemical and biophysical research communications, Vol. 293, Issue 5, pp. 1364-9, 2002