anti-Mouse (Murine) TLR3 antibody for Functional Studies

Recommended TLR3 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 3 (TLR3) Antibodies
  • CD283
  • IIAE2
  • AI957183
  • zgc:123006
  • toll like receptor 3
  • toll-like receptor 3
  • TLR3
  • Tlr3
  • tlr3
Reactivity
Dog (Canine), Human, Mouse (Murine)
276
206
64
44
5
4
3
2
2
2
1
1
1
1
1
Host
Mouse
198
119
39
3
3
Clonality
Monoclonal
Conjugate
This TLR3 antibody is conjugated to FITC
34
29
24
16
10
8
7
6
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
234
140
103
88
64
42
40
33
13
13
12
7
4
4
1
1
Supplier
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Catalog No. ABIN1106214
$ 489.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106212 Func IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 Log in to see TLR3-7 5
1 ABIN1106213 Func IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 Log in to see TLR3-7 4

General

Antigen Toll-Like Receptor 3 (TLR3) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine)
(276), (206), (64), (44), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(198), (119), (39), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TLR3 antibody is conjugated to FITC
(34), (29), (24), (16), (10), (8), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(234), (140), (103), (88), (64), (42), (40), (33), (13), (13), (12), (7), (4), (4), (1), (1)
Pubmed 4 references available
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Product Details anti-TLR3 Antibody

Target Details TLR3 Application Details Handling References for anti-TLR3 antibody (ABIN1106214) Images
Specificity The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283).
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, canine
Purification Protein G
Immunogen Human Flag-tagged TLR3 stably expressed by Ba/F3 cells
Clone TLR3-7
Isotype IgG1
Plasmids, Primers & others

Target Details TLR3

Product Details anti-TLR3 Antibody Application Details Handling References for anti-TLR3 antibody (ABIN1106214) Images back to top
Antigen
Alternative Name CD283 / TLR3 (TLR3 Antibody Abstract)
Background Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.Synonyms: Toll-like receptor 3
Gene ID 7098
NCBI Accession NP_003256
UniProt O15455
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR3 Antibody Target Details TLR3 Handling References for anti-TLR3 antibody (ABIN1106214) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details References for anti-TLR3 antibody (ABIN1106214) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.02 % sodium azide, 1 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.

References for anti-TLR3 antibody (ABIN1106214)

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details Handling Images back to top
Background publications

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Oshiumi, Matsumoto, Funami, Akazawa, Seya: "TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction." in: Nature immunology, Vol. 4, Issue 2, pp. 161-7, 2003

Matsumoto, Funami, Tanabe, Oshiumi, Shingai, Seto, Yamamoto, Seya: "Subcellular localization of Toll-like receptor 3 in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 3154-62, 2003

Matsumoto, Kikkawa, Kohase, Miyake, Seya: "Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling." in: Biochemical and biophysical research communications, Vol. 293, Issue 5, pp. 1364-9, 2002