anti-Cow (Bovine) NUP50 antibody for Immunofluorescence

Recommended NUP50 Antibody (supplied by: Log in to see )

Antigen
Nucleoporin 50kDa (NUP50) Antibodies
  • wu:fa56e03
  • wu:fb78a08
  • GB18194
  • npap60
  • npap60l
  • Nup50
  • Rtp60
  • 1700030K07Rik
  • AI413123
  • Npap60
  • NPAP60
  • NPAP60L
  • nucleoporin 50
  • nuclear pore complex protein Nup50
  • nucleoporin 50kDa S homeolog
  • nucleoporin 50kDa
  • nup50
  • LOC410864
  • NUP50
  • nup50.S
  • CpipJ_CPIJ012737
  • Nup50
Epitope
C-Term
21
11
9
8
7
5
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae)
107
40
28
8
2
2
2
1
1
1
Host
Rabbit
74
19
19
Clonality
Polyclonal
Conjugate
This NUP50 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
93
55
20
13
13
9
5
4
4
2
Options
Supplier
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Available images

Catalog No. ABIN2784862
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1919047 IF IHC IHC (p) IP WB Rabbit IgG AA 418-468 Log in to see Polyclonal 0
1 ABIN1919048 IF IHC IHC (p) Rabbit IgG AA 418-468 Log in to see Polyclonal 0

General

Antigen Nucleoporin 50kDa (NUP50) Antibodies
Epitope C-Term
(21), (11), (9), (8), (7), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae)
(107), (40), (28), (8), (2), (2), (2), (1), (1), (1)
Host Rabbit
(74), (19), (19)
Clonality
Polyclonal
Conjugate This NUP50 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(93), (55), (20), (13), (13), (9), (5), (4), (4), (2)
Pubmed 1 reference available
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Product Details anti-NUP50 Antibody

Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN2784862) Images
Blocking Peptide Blocking peptide for this product available: ABIN981035
Sequence TTQSKPVSSP FPTKPLEGQA EGDSGECKGG DEEENDEPPK VVVTEVKEED
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 91%, Pig: 100%, Rabbit: 100%, Rat: 92%, Yeast: 80%
Characteristics This is a rabbit polyclonal antibody against NUP50. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NUP50
Plasmids, Primers & others

Target Details NUP50

Product Details anti-NUP50 Antibody Application Details Handling References for anti-NUP50 antibody (ABIN2784862) Images back to top
Antigen
Alternative Name NUP50 (NUP50 Antibody Abstract)
Background The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: MGC39961, NPAP60, NPAP60L
Protein Interaction Partner: KPNA5, KPNA4, KPNA3, KPNA2, KPNA1, KPNA6, UBC, KIAA1033, SART3, TTI1, TSC22D1, IKBKAP, KIF5B, EPB41L1, ACACA, SRPK2, LMNA, HDAC6, NPM1, UBAP2, TOR1AIP1, NUP153, APP, CUL3, Mki67, Kifc5b, SLX4, KPNB1, XPO1, RAN, CDKN1B, IPO13,
Protein Size: 468
Molecular Weight 50 kDa
Gene ID 10762
NCBI Accession NM_007172, NP_009103
UniProt Q9UKX7
Pathways Tube Formation

Application Details

Product Details anti-NUP50 Antibody Target Details NUP50 Handling References for anti-NUP50 antibody (ABIN2784862) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 468 AA

Restrictions For Research Use only

Handling

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details References for anti-NUP50 antibody (ABIN2784862) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NUP50 antibody (ABIN2784862)

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN2784862) back to top
Supplier Images
Western Blotting (WB) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2784862) WB Suggested Anti-NUP50 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell l...