anti-Human NUP50 antibody for Immunocytochemistry

Recommended NUP50 Antibody

Antigen
Nucleoporin 50kDa (NUP50) Antibodies
  • wu:fa56e03
  • wu:fb78a08
  • GB18194
  • npap60
  • npap60l
  • Nup50
  • Rtp60
  • 1700030K07Rik
  • AI413123
  • Npap60
  • NPAP60
  • NPAP60L
  • nucleoporin 50
  • nuclear pore complex protein Nup50
  • nucleoporin 50kDa S homeolog
  • nucleoporin 50kDa
  • nup50
  • LOC410864
  • NUP50
  • nup50.S
  • CpipJ_CPIJ012737
  • Nup50
Epitope
C-Term
20
10
8
8
7
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
111
39
29
9
3
3
3
2
2
1
1
Host
Rabbit
79
19
18
Clonality
Polyclonal
Conjugate
This NUP50 antibody is un-conjugated
6
5
5
4
4
4
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
55
20
13
12
9
5
4
3
2
Options

Available images

Catalog No. ABIN2856374
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19 ABIN2119046 ICC IHC WB Rabbit IgG AA 37-269 Polyclonal 0
4.946251 ABIN4341181 ICC IF IHC IHC (p) WB Rabbit IgG Polyclonal 0
-1.0537491 ABIN4341182 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
-2.553749 ABIN408603 ICC IF IHC IHC (p) IP WB Rabbit AA 418-468 Polyclonal 1
-4.053749 ABIN5078482 ICC IF WB Rabbit IgG Polyclonal 0

General

Antigen Nucleoporin 50kDa (NUP50) Antibodies
Epitope C-Term
(20), (10), (8), (8), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(111), (39), (29), (9), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(79), (19), (18)
Clonality
Polyclonal
Conjugate This NUP50 antibody is un-conjugated
(6), (5), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (55), (20), (13), (12), (9), (5), (4), (3), (2)
Supplier

Product Details anti-NUP50 Antibody

Target Details NUP50 Application Details Handling Images
Cross-Reactivity Xenopus laevis, Xenopus tropicalis, Chicken, Rhesus Monkey, Cow (Bovine)
Cross-Reactivity (Details) Xenopus laevis (91 %), Xenopus Tropicalis (91 %), Chicken (92 %), Rhesus Monkey (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to NUP50 (nucleoporin 50 kDa)
NUP50 antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details NUP50

Product Details anti-NUP50 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NUP50 (NUP50 Antibody Abstract)
Background The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus , nuclear pore complex , Nucleus membrane, Peripheral membrane protein, Nucleoplasmic side (By similarity)
Molecular Weight 50 kDa
Gene ID 10762
Pathways Tube Formation

Application Details

Product Details anti-NUP50 Antibody Target Details NUP50 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: H1299 , mouse testis

Restrictions For Research Use only

Handling

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Images back to top
Format Liquid
Concentration 0.79 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) IHC-P Image Immunohistochemical analysis of paraffin-embedded human normal colon, usi...
Western Blotting (WB) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) WB Image Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE antibody diluted a...
Western Blotting (WB) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) WB Image NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis. ...
Immunofluorescence (IF) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...