anti-Human Adenomatous Polyposis Coli antibody for Immunofluorescence

Recommended Adenomatous Polyposis Coli Antibody (supplied by: Log in to see )

Antigen
Adenomatous Polyposis Coli (APC) Antibodies
  • dp2
  • dp3
  • fap
  • fpc
  • xapc
  • dp2.5
  • AI047805
  • AU020952
  • AW124434
  • CC1
  • Min
  • mAPC
  • BTPS2
  • DP2
  • DP2.5
  • DP3
  • GS
  • PPP1R46
  • RATAPC
  • APC1
  • APC, WNT signaling pathway regulator
  • adenomatous polyposis coli
  • adenomatous polyposis coli protein, putative
  • adenomatosis polyposis coli
  • adenomatous polyposis coli L homeolog
  • APC
  • apc
  • Smp_139190
  • Apc
  • apc.L
Epitope
AA 2744-2845
29
21
20
18
15
9
9
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
176
106
76
10
7
6
5
4
2
2
2
1
Host
Mouse
164
34
15
2
1
Clonality
Monoclonal
Conjugate
Un-conjugated
10
10
7
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
149
107
62
50
46
29
26
20
18
7
4
2
1
1
1
Options
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Catalog No. ABIN393899
$ 527.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.463114 ABIN3183309 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
14.728937 ABIN2704484 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
14.728937 ABIN559888 IF PLA ELISA WB Mouse IgG1 kappa AA 2744-2843 Log in to see 3E2 0
12.463114 ABIN5618818 ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
10 ABIN6255083 ELISA ICC IF IHC WB Rabbit IgG pSer2054 Log in to see Polyclonal 0
10 ABIN6259974 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
8.185528 ABIN4280975 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 1
7 ABIN265313 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN5960388 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1827364 IF ELISA WB Mouse IgG1, kappa AA 2744-2843 Log in to see 3E2 0
1 ABIN2891311 IF IHC IHC (p) WB Rabbit Gln2829 Log in to see Polyclonal 0
1 ABIN2616774 ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1893368 IF IHC IHC (p) Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN1893367 IF IHC IHC (p) IP Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN1893369 IF IHC IHC (p) IP Rabbit IgG AA 75-125 Log in to see Polyclonal 0
1 ABIN1893370 IF IHC IHC (p) IP Rabbit IgG AA 2793-2843, C-Term Log in to see Polyclonal 0
1 ABIN2616772 ELISA IF WB Rat IgG2a Log in to see T45 0
1 ABIN2616771 ELISA IF WB Rat IgG2a Log in to see 0
1 ABIN2202072 IF ELISA WB Mouse IgG1 kappa AA 2744-2843 Log in to see 3E2 0
0.6855283 ABIN4280974 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0

General

Antigen Adenomatous Polyposis Coli (APC) Antibodies
Epitope AA 2744-2845
(29), (21), (20), (18), (15), (9), (9), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(176), (106), (76), (10), (7), (6), (5), (4), (2), (2), (2), (1)
Host Mouse
(164), (34), (15), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(10), (10), (7), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(149), (107), (62), (50), (46), (29), (26), (20), (18), (7), (4), (2), (1), (1), (1)
Pubmed 6 references available
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Product details

Target Details Application Details Handling References Images
Specificity APC (NP_000029, 2744 a.a. ~ 2845 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: APC (NP_000029, 2744 a.a. ~ 2843 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 3E2
Isotype IgG1 kappa

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name APC (APC Antibody Abstract)
Background

Other Names: Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 25, APC, DP25

Gene Description: adenomatous polyposis coli

Gene Summary: This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq

Molecular Weight 311646 DA
Gene ID 324
NCBI Accession NP_000029, NP_001120982
UniProt P25054
Pathways WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western blotting=1:500-1000
Comment

Background: This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References

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Product cited in:

Liu, Song, Luan, Sun, Bai, Wang, Li, Zhang, Feng, Du: "Promoter methylation status of tumor suppressor genes and inhibition of expression of DNA methyltransferase 1 in non-small cell lung cancer." in: Experimental biology and medicine (Maywood, N.J.), Vol. 241, Issue 14, pp. 1531-9, 2017 Method employed by authors: Western Blotting (WB)

Background publications

Jaulin, Kreitzer: "KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC." in: The Journal of cell biology, Vol. 190, Issue 3, pp. 443-60, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Lee, Kim, Ku, Kim, Choi, Kim: "Association between polymorphisms in Wnt signaling pathway genes and bone mineral density in postmenopausal Korean women." in: Menopause (New York, N.Y.), Vol. 17, Issue 5, pp. 1064-70, 2010

Wish, Hyde, Parfrey, Green, Younghusband, Simms, Fontaine, Dicks, Stuckless, Gallinger, McLaughlin, Woods, Green: "Increased cancer predisposition in family members of colorectal cancer patients harboring the p.V600E BRAF mutation: a population-based study." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 7, pp. 1831-9, 2010

Albuquerque, Baltazar, Filipe, Penha, Pereira, Smits, Cravo, Lage, Fidalgo, Claro, Rodrigues, Veiga, Ramos, Fonseca, Leitão, Fodde: "Colorectal cancers show distinct mutation spectra in members of the canonical WNT signaling pathway according to their anatomical location and type of genetic instability." in: Genes, chromosomes & cancer, Vol. 49, Issue 8, pp. 746-59, 2010

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Adenomatous Polyposis Coli (APC) (AA 2744-2845) antibody (ABIN393899) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Immunofluorescence (IF) image for anti-Adenomatous Polyposis Coli (APC) (AA 2744-2845) antibody (ABIN393899) Immunofluorescence of monoclonal antibody to APC on HeLa cell . [antibody concentrati...
ELISA image for anti-Adenomatous Polyposis Coli (APC) (AA 2744-2845) antibody (ABIN393899) Detection limit for recombinant GST tagged APC is approximately 30ng/ml as a capture ...