anti-Human Adenomatous Polyposis Coli antibody for Immunohistochemistry (Frozen Sections)

Recommended Adenomatous Polyposis Coli Antibody (supplied by: Log in to see )

Antigen
Adenomatous Polyposis Coli (APC) Antibodies
  • dp2
  • dp3
  • fap
  • fpc
  • xapc
  • dp2.5
  • AI047805
  • AU020952
  • AW124434
  • CC1
  • Min
  • mAPC
  • BTPS2
  • DP2
  • DP2.5
  • DP3
  • GS
  • PPP1R46
  • RATAPC
  • APC1
  • APC, WNT signaling pathway regulator
  • adenomatous polyposis coli
  • adenomatous polyposis coli protein, putative
  • adenomatosis polyposis coli
  • adenomatous polyposis coli L homeolog
  • APC
  • apc
  • Smp_139190
  • Apc
  • apc.L
Epitope
AA 2634-2843
29
21
20
18
15
9
9
5
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
176
106
76
10
7
6
5
4
2
2
2
1
Host
Rabbit
163
35
15
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
7
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
149
107
61
49
47
28
26
19
18
7
4
2
1
1
1
Options
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Catalog No. ABIN1858037
$ 350.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN224804 IHC IHC (fro) WB Mouse IgG1 AA 1-433 Log in to see 0
-5.54311 ABIN153070 ICC IHC (fro) IF IHC (p) IHC Mouse IgG2b Log in to see CC-1 1
-8.54311 ABIN152091 IHC (fro) IP IHC WB Mouse IgG1 N-Term Log in to see ALi 12-28 2
-13.04311 ABIN5950996 IHC IHC (fro) Janelia Fluor® 549 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN5950997 IHC IHC (fro) Janelia Fluor® 646 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280993 IHC IHC (fro) WB Alexa Fluor 488 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280994 IHC IHC (fro) WB Alexa Fluor 647 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280995 IHC IHC (fro) WB Alexa Fluor 700 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4281000 IHC IHC (fro) WB DyLight 680 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280999 IHC IHC (fro) FITC Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280992 IHC IHC (fro) WB Alexa Fluor 405 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280997 IHC IHC (fro) WB Biotin Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4281001 IHC IHC (fro) WB DyLight 488 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4281006 IHC IHC (fro) WB DyLight 350 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4281005 IHC IHC (fro) WB DyLight 550 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4281007 IHC IHC (fro) WB DyLight 405 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4280998 IHC IHC (fro) WB DyLight 650 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4281002 IHC IHC (fro) WB DyLight 755 Mouse IgG1 N-Term Log in to see ALi 12-28 0
-13.04311 ABIN4254152 IHC IHC (fro) WB HRP Mouse IgG1 N-Term Log in to see ALi 12-28 0

General

Antigen Adenomatous Polyposis Coli (APC) Antibodies
Epitope AA 2634-2843
(29), (21), (20), (18), (15), (9), (9), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(176), (106), (76), (10), (7), (6), (5), (4), (2), (2), (2), (1)
Host Rabbit
(163), (35), (15), (2), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (10), (7), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(149), (107), (61), (49), (47), (28), (26), (19), (18), (7), (4), (2), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-ATNGAES KTLIYQMAPA VSKTEDVWVR IEDCPINNPR SGRSPTGNTP PVIDSVSEKA NPNIKDSKDN QAKQNVGNGS VPMRTVGLEN RLNSFIQVDA PDQKGTEIKP GQNNPVPVSE TNESSIVERT PFSSSSSSKH SSPSGTVAAR VTPFNYNPSP RKSSADSTSA RPSQIPTPVN NNTKKRDSKT DSTESSGTQS PKRHSGSYLV TSV
Specificity It has been selected for its ability to recognize APC in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen APC (AA 2634-2843)
Isotype IgG

Target Details

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Antigen
Alternative Name Adenomatosis Polyposis Coli Protein (APC) (APC Antibody Abstract)
Pathways WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization

Application Details

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Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant APC (Ala2634~Val2843) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Adenomatous Polyposis Coli (APC) (AA 2634-2843) antibody (ABIN1858037) anti-Adenomatous Polyposis Coli (APC) (AA 2634-2843) antibody
 image for anti-Adenomatous Polyposis Coli (APC) (AA 2634-2843) antibody (ABIN1858037) Figure.DAB staining on IHC-P. Samples: Human Tissue