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|Antigen||Adenomatous Polyposis Coli (APC) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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A synthetic peptide derived from C-terminus of human APC protein.
Type of Immunogen: Synthetic peptide
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|Alternative Name||APC (APC Antibody Abstract)|
Name/Gene ID: APC
Synonyms: APC, Adenomatosis polyposis coli, Adenomatous polyposis coli, BTPS2, Deleted in polyposis 2.5, DP2, DP2.5, FPC, GS, Protein APC, PPP1R46, DP3
|Pathways||WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization|
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Approved: IHC-P (10 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
|Restrictions||For Research Use only|
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|Buffer||10 mM PBS, pH 7.4, BSA, sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||2-8 °C.|
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