anti-Pig (Porcine) DKK1 antibody for Immunohistochemistry

Recommended DKK1 Antibody (supplied by: Log in to see )

Antigen
Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) Antibodies
  • DKK-1
  • SK
  • dkk1
  • dkk
  • dkk-1
  • xdkk1
  • Xdkk-1
  • Dickkopf1
  • dickkopf-1
  • mdkk-1
  • dkk1-A
  • dickkopf WNT signaling pathway inhibitor 1
  • dickkopf-related protein 1
  • dickkopf WNT signaling pathway inhibitor 1b
  • dickkopf protein
  • dickkopf WNT signaling pathway inhibitor 1 S homeolog
  • DKK1
  • Dkk1
  • LOC100228789
  • dkk1b
  • dkk1
  • dickkopf
  • dkk1.S
Epitope
Internal Region
35
26
18
15
12
11
8
8
8
6
6
6
5
5
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Horse (Equine), Human, Monkey, Pig (Porcine)
235
63
57
25
8
8
7
7
5
4
4
4
3
2
2
2
1
1
1
Host
Rabbit
190
41
21
2
Clonality
Polyclonal
Conjugate
This DKK1 antibody is un-conjugated
13
11
8
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
193
105
49
45
13
10
10
7
5
4
3
1
1
1
1
1
Supplier
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Catalog No. ABIN1048502
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.312949 ABIN2469324 IHC Rabbit N-Term Log in to see Polyclonal 0
1 ABIN296913 ELISA IHC IHC (p) WB Goat AA 250-262 Log in to see Polyclonal 0
1 ABIN213403 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) Antibodies
Epitope Internal Region
(35), (26), (18), (15), (12), (11), (8), (8), (8), (6), (6), (6), (5), (5), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Horse (Equine), Human, Monkey, Pig (Porcine)
(235), (63), (57), (25), (8), (8), (7), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(190), (41), (21), (2)
Clonality
Polyclonal
Conjugate This DKK1 antibody is un-conjugated
(13), (11), (8), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(193), (105), (49), (45), (13), (10), (10), (7), (5), (4), (3), (1), (1), (1), (1), (1)
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Product Details anti-DKK1 Antibody

Target Details DKK1 Application Details Handling Images
Brand IHC-plus™
Specificity Human DKK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Bat, Horse, Pig (100%) Galago, Sheep, Goat, Elephant, Bovine, Rabbit, Guinea pig (94%) Hamster, Panda, Dog (88%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human DKK1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Bat, Horse, Pig (100%), Galago, Sheep, Goat, Elephant, Bovine, Rabbit, Guinea pig (94%), Hamster, Panda, Dog (88%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details DKK1

Product Details anti-DKK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name DKK1 (DKK1 Antibody Abstract)
Background Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK
Gene ID 22943
Pathways WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-DKK1 Antibody Target Details DKK1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (10 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-DKK1 Antibody Target Details DKK1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-DKK1 Antibody Target Details DKK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) (Internal Region) antibody (ABIN1048502) Anti-DKK1 antibody ABIN1048502 IHC staining of human uterus, endometrium. Immunohist...