anti-Human FZD4 antibody for Cytometry by Time of Flight

Recommended FZD4 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 4 (FZD4) Antibodies
  • CG4626
  • DFz4
  • Dfz4
  • Dm Fz4
  • Dmel\\CG4626
  • Fz4
  • anon-WO0170980.10
  • anon-WO0170980.11
  • fz1
  • zg01
  • CD344
  • EVR1
  • FEVR
  • FZD4S
  • Fz-4
  • FzE4
  • GPCR
  • hFz4
  • frizzled4
  • fz4
  • FZ-4
  • frizzled 4
  • frizzled class receptor 4
  • frizzled-4
  • frizzled class receptor 4 S homeolog
  • fz4
  • fzd4
  • Tsp_10376
  • FZD4
  • Fzd4
  • fzd4.S
Epitope
AA 37-180
36
26
16
15
10
9
8
8
4
4
4
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
144
117
60
8
7
7
7
6
5
5
5
4
3
2
2
1
Host
Rat
131
26
21
8
Clonality
Monoclonal
Conjugate
This FZD4 antibody is un-conjugated
11
8
7
7
6
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
117
67
24
20
18
13
12
6
4
2
1
1
1
Supplier
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Catalog No. ABIN4900304
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General

Antigen Frizzled Family Receptor 4 (FZD4) Antibodies
Epitope AA 37-180
(36), (26), (16), (15), (10), (9), (8), (8), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(144), (117), (60), (8), (7), (7), (7), (6), (5), (5), (5), (4), (3), (2), (2), (1)
Host Rat
(131), (26), (21), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FZD4 antibody is un-conjugated
(11), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(117), (67), (24), (20), (18), (13), (12), (6), (4), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FZD4 Antibody

Target Details FZD4 Application Details Handling References for anti-FZD4 antibody (ABIN4900304) Images
Specificity Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4, Phe37-Glu180, Accession # Q61088
Clone 145901
Isotype IgG2a
Plasmids, Primers & others

Target Details FZD4

Product Details anti-FZD4 Antibody Application Details Handling References for anti-FZD4 antibody (ABIN4900304) Images back to top
Antigen
Alternative Name Frizzled-4 (FZD4 Antibody Abstract)
Background Alternative Names: CD344, CD344 antigen, EVR1, exudative vitreoretinopathy 1, FEVR, frizzled (Drosophila) homolog 4, frizzled homolog 4 (Drosophila), frizzled-4, Frizzled4, Fz-4, FZD4, FZD4S, FzE4, GPCR, hFz4, MGC34390, WNT receptor frizzled-4
Gene ID 8322
UniProt Q61088
Pathways WNT Signaling, Hormone Transport, Sensory Perception of Sound

Application Details

Product Details anti-FZD4 Antibody Target Details FZD4 Handling References for anti-FZD4 antibody (ABIN4900304) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details References for anti-FZD4 antibody (ABIN4900304) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-FZD4 antibody (ABIN4900304)

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling Images back to top
Product cited in:

Baksh, Boland, Tuan: "Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation." in: Journal of cellular biochemistry, Vol. 101, Issue 5, pp. 1109-24, 2007 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Chen, Park, Tang: "Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation." in: The Journal of biological chemistry, Vol. 281, Issue 17, pp. 11910-6, 2006 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).