anti-Human LEF1 antibody for Western Blotting

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer-binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • lef1
  • Lef1
Epitope
Middle Region
15
13
13
9
8
6
6
6
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
132
41
29
10
7
6
6
5
4
4
3
3
3
2
1
1
Host
Rabbit
57
55
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
122
68
25
20
9
9
4
3
2
2
2
1
1
1
1
Options
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Catalog No. ABIN2779621
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2596397 ABIN2792635 WB Rabbit N-Term Log in to see Polyclonal 1
3.2596397 ABIN2779620 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.2596397 ABIN655987 IF WB Rabbit Ig Fraction AA 10-37, N-Term Log in to see Polyclonal 4
3.2596397 ABIN735248 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
3.2596397 ABIN4330626 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.2596397 ABIN1531685 ELISA WB Rabbit IgG AA 8-57, pSer42 Log in to see Polyclonal 0
3.2596397 ABIN565556 RNAi ELISA WB Mouse IgG1 kappa AA 1-399, full length Log in to see 3H5 2
3.2596397 ABIN264381 IF WB Mouse IgG1 HMG Binding Domain Log in to see REMB6 1
3.2596397 ABIN188546 ELISA WB Goat Log in to see Polyclonal 2
3.2596397 ABIN526527 IF ELISA WB Mouse IgG1 kappa AA 14-123, partial Log in to see 2C9 0
3.2596397 ABIN526526 IF ELISA WB Mouse IgG1 kappa AA 14-123, partial Log in to see 5A3 0
3.2596397 ABIN526528 ELISA WB Mouse IgG1 kappa AA 14-123, partial Log in to see 1H2 0
3.2596397 ABIN126828 EIA WB Mouse IgG1 Log in to see 2D12 0
3.2596397 ABIN264397 WB Mouse IgG1 Alternate Exon Log in to see 1C3 0
3.2596397 ABIN1882259 WB Mouse IgG1 AA 10-37 Log in to see 661CT8-3-5 3
3.2596397 ABIN396506 IF WB Mouse IgG1 kappa AA 14-124 Log in to see 5A3 0
3.2596397 ABIN395469 IF WB Mouse IgG1 kappa AA 14-124 Log in to see 2C9 0
3.2596397 ABIN526525 WB Mouse AA 1-399, full length Log in to see Polyclonal 0
3.2596397 ABIN2460133 ELISA WB Rabbit Log in to see Polyclonal 0
3.2596397 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal 0

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope Middle Region
(15), (13), (13), (9), (8), (6), (6), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(132), (41), (29), (10), (7), (6), (6), (5), (4), (4), (3), (3), (3), (2), (1), (1)
Host Rabbit
(57), (55), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(122), (68), (25), (20), (9), (9), (4), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN979264
Sequence ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG
Predicted Reactivity Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 86%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 79%
Characteristics This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LEF1

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1).
Alias Symbols: DKFZp586H0919, FLJ46390, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399
Molecular Weight 44 kDa
Gene ID 51176
NCBI Accession NM_016269, NP_057353
UniProt Q9UJU2
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 399 AA

Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Middle Region) antibody (ABIN2779621) WB Suggested Anti-LEF1 Antibody Titration: 0.1-2.0ug/ml
Positive Control...
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Middle Region) antibody (ABIN2779621) Host: Rabbit
Target Name: LEF1
Sample Tissue: Human Fetal Heart
...