Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody

Details for Product No. ABIN272048, Supplier: Log in to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Reactivity
Human, Mouse (Murine)
117
57
44
7
7
5
4
4
3
3
Host
Rabbit
90
20
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
75
41
33
26
22
5
4
3
3
2
2
1
Options
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Specificity This antibody detects endogenous levels of p40 phox protein. (region surrounding Arg149)
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE
Alternative Name NCF-4 (NCF4 Antibody Abstract)
Background Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase. However, p40-phox appears to downregulate oxidase function as well, by competing with an SH3 domain interaction between other essential oxidase components.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox
Molecular Weight approx. 39 kDa
Gene ID 4689
NCBI Accession NP_000622
UniProt Q15080
Application Notes ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody (ABIN272048) anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody