Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (C-Term) antibody

Details for Product No. ABIN2787877, Supplier: Log in to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Epitope
C-Term
19
9
8
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
117
58
44
7
7
5
4
4
3
3
Host
Rabbit
90
20
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
74
41
33
26
23
5
4
3
3
2
2
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NCF4
Blocking Peptide Blocking peptide for this product available: ABIN980524
Sequence PSLRPLPLTS PSHGSLSHSK APSGSQMSHN AVTSHQRPGW PGQPHSPFPH
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against NCF4. It was validated on Western Blot.
Purification Affinity Purified
Alternative Name NCF4 (NCF4 Antibody Abstract)
Background NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: MGC3810, NCF, P40PHOX, SH3PXD4
Protein Interaction Partner: NCF2, UBC, CORO1A, MSN, CYBB, NCF1, CYBA, TXN, PRKCD, XRCC6, PRKDC,
Protein Size: 348
Molecular Weight 39 kDa
Gene ID 4689
NCBI Accession NM_013416, NP_038202
UniProt A8K4F9
Research Area Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 348 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (C-Term) antibody (ABIN2787877) WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (C-Term) antibody (ABIN2787877) WB Suggested Anti-NCF4
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500 ...