NCF4 antibody (C-Term)
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- Target See all NCF4 Antibodies
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NCF4 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- PSLRPLPLTS PSHGSLSHSK APSGSQMSHN AVTSHQRPGW PGQPHSPFPH
- Predicted Reactivity
- Human: 100%
- Characteristics
- This is a rabbit polyclonal antibody against NCF4. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the C terminal region of human NCF4
- Top Product
- Discover our top product NCF4 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 348 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- Alternative Name
- NCF4 (NCF4 Products)
- Synonyms
- p40phox antibody, AI451400 antibody, NCF antibody, P40PHOX antibody, SH3PXD4 antibody, neutrophil cytosolic factor 4 antibody, ncf4 antibody, Ncf4 antibody, NCF4 antibody
- Background
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NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: MGC3810, NCF, P40PHOX, SH3PXD4
Protein Interaction Partner: NCF2, UBC, CORO1A, MSN, CYBB, NCF1, CYBA, TXN, PRKCD, XRCC6, PRKDC,
Protein Size: 348 - Molecular Weight
- 39 kDa
- Gene ID
- 4689
- NCBI Accession
- NM_013416, NP_038202
- UniProt
- A8K4F9
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