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BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 92-127)

Details for Product anti-BAD Antibody No. ABIN388118, Supplier: Log in to see
Antigen
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
Alternatives
anti-Mouse (Murine) BAD antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 92-127, BH3 Domain
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46
41
37
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34
27
27
24
20
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Reactivity
Human, Mouse (Murine)
715
369
212
10
7
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1
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Host
Rabbit
908
48
2
1
Clonality (Clone)
Polyclonal ()
Conjugate
This BAD antibody is un-conjugated
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65
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59
11
6
6
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6
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
509
418
415
279
72
59
46
23
7
4
4
3
2
2
1
1
1
Supplier
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Immunogen This Bad BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-127 AA from human Bad BH3 Domain.
Clone RB4617
Isotype Ig
Specificity This Bad BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 100-130 (BH3 domain) of human Bad.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Bad (BAD Antibody Abstract)
Background Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.
Synonyms: Bcl2-antagonist of cell death, BAD, Bcl-2 binding component 6, Bcl-XL/Bcl-2 associated death promoter, Bcl-2-like 8 protein
Molecular Weight 18392 DA
Gene ID 572
UniProt Q92934
Research Area Apoptosis/Necrosis, Cell Signaling, Cell Structure, Cancer
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 92-127) (ABIN388118) Western blot analysis of Bad BH3 Domain antibody (ABIN388118) in mouse bladder tissue...
Immunohistochemistry (IHC) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 92-127) (ABIN388118) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Won, Kim, La et al.: "Cleavage of 14-3-3 protein by caspase-3 facilitates bad interaction with Bcl-x(L) during apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 21, pp. 19347-51, 2003 (PubMed).

Cowburn, Cadwallader, Reed et al.: "Role of PI3-kinase-dependent Bad phosphorylation and altered transcription in cytokine-mediated neutrophil survival." in: Blood, Vol. 100, Issue 7, pp. 2607-16, 2002 (PubMed).

Mabuchi, Ohmichi, Kimura et al.: "Inhibition of phosphorylation of BAD and Raf-1 by Akt sensitizes human ovarian cancer cells to paclitaxel." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33490-500, 2002 (PubMed).