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anti-Mouse (Murine) Caspase 4 antibody for Enzyme Immunoassay

Recommended Caspase 4 Antibody (supplied by: Log in to see )

Antigen
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies
  • CASP1
  • CASP4
  • Casp11
  • CASP13
  • Caspl
  • ICE(rel)II
  • ICEREL-II
  • ICH-2
  • ich-3
  • Mih1/TX
  • TX
Epitope
N-Term
44
26
14
10
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
168
93
31
20
2
1
1
Host
Rabbit
185
30
22
15
6
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
14
11
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
213
105
74
47
32
29
27
24
19
11
8
3
3
1
1
1
Supplier
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Catalog No. ABIN499561
$ 401.50
Plus shipping costs $45.00

Top referenced anti-Caspase 4 antibody for Enzyme Immunoassay

Similar anti-Caspase 4 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 43 Antibodies 59 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 24 Antibodies 8 Antibodies
Immunofluorescence (IF) 21 Antibodies 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Microarray (MA) 18 Antibodies
Western Blotting (WB) 73 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies
Epitope N-Term
(44), (26), (14), (10), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(168), (93), (31), (20), (2), (1), (1)
Host Rabbit
(185), (30), (22), (15), (6)
Clonality
Polyclonal
Conjugate This Caspase 4 antibody is un-conjugated
(14), (11), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(213), (105), (74), (47), (32), (29), (27), (24), (19), (11), (8), (3), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Caspase 4 Antibody

Target Details Caspase 4 Application Details Handling References for anti-Caspase 4 Antibody (ABIN499561) Images
Specificity This antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.
Purification Affinity chromatography purified via peptide column
Immunogen Human Casp-4 / ICH-2 / ICE rel II / Mih1 (N-Terminus) Peptide
Isotype IgG

Target Details Caspase 4

Product Details anti-Caspase 4 Antibody Application Details Handling References for anti-Caspase 4 Antibody (ABIN499561) Images back to top
Antigen
Alternative Name Caspase-4 (CASP4 Antibody Abstract)
Background Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease (5).Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX
Gene ID 837
UniProt P49662
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Handling References for anti-Caspase 4 Antibody (ABIN499561) Images back to top
Application Notes ELISA. Western Blot: Casp-4 antibody can be used for the detection of Caspase-4 at 1 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details References for anti-Caspase 4 Antibody (ABIN499561) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Caspase 4 Antibody (ABIN499561)

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Handling Images back to top
Background publications

Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004

Gracie, Robertson, McInnes: "Interleukin-18." in: Journal of leukocyte biology, Vol. 73, Issue 2, pp. 213-24, 2003

Kamens, Paskind, Hugunin, Talanian, Allen, Banach, Bump, Hackett, Johnston, Li: "Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15250-6, 1995

Kuida, Lippke, Ku, Harding, Livingston, Su, Flavell: "Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 267, Issue 5206, pp. 2000-3, 1995

Images

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Handling References for anti-Caspase 4 Antibody (ABIN499561) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN499561) Immunofluorescence of Caspase-4 in Mouse Spleen 100 cells with Caspase-4 Antibody  at...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN499561) Immunohistochemical staining of mouse spleen using Caspase-4 antibody at 2 μg/ml.
Western Blotting (WB) image for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN499561) Western blot analysis of Caspase-4 in Ramos cells with Caspase-4 antibody at (A) 0.5 ...