anti-Human Integrin beta 2 antibody for Western Blotting

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Epitope
AA 720-769, pThr758
46
34
26
23
16
15
14
14
12
5
5
5
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
478
212
117
43
38
21
21
13
5
4
3
3
3
3
3
2
1
1
Host
Rabbit
364
165
161
10
3
1
Clonality
Polyclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
88
73
52
27
15
12
9
8
8
8
8
6
6
6
6
6
6
5
5
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
485
286
203
147
95
89
76
68
52
40
26
17
11
9
7
6
4
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN1531540
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0849469 ABIN2705756 WB Rabbit Center Log in to see Polyclonal
3.0849469 ABIN3043260 ICC IHC (p) WB Rabbit IgG AA 404-769 Log in to see Polyclonal
3.0849469 ABIN2749020 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 10
3.0849469 ABIN2749019 Func IHC (f) FACS IP IHC (p) WB Mouse IgG1 Log in to see MEM-48 12
3.0849469 ABIN125688 FACS Func IHC (f) IHC (p) IP WB APC Mouse IgG1 AA 534-546 Log in to see MEM-48 19
3.0849469 ABIN192094 FACS Func IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 534-546 Log in to see MEM-48 20
3.0849469 ABIN94007 FACS Func IHC (f) IHC (p) IP WB Biotin Mouse IgG1 AA 534-546 Log in to see MEM-48 20
3.0849469 ABIN94008 FACS Func IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 534-546 Log in to see MEM-48 20
3.0849469 ABIN94005 FACS Func IHC (f) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
3.0849469 ABIN1882257 WB Mouse IgG1 Log in to see 534CT6-2-1 5
3.0849469 ABIN192259 FACS Func IP WB PE Mouse IgG1 Log in to see MEM-148 10
3.0849469 ABIN125714 FACS Func IP WB APC Mouse IgG1 Log in to see MEM-148 10
3.0849469 ABIN94010 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 8
3.0849469 ABIN3044225 IHC (p) WB Rabbit IgG AA 732-748, C-Term Log in to see Polyclonal
3.0849469 ABIN3043698 IHC (p) WB Rabbit IgG AA 24-58, N-Term Log in to see Polyclonal
3.0849469 ABIN269112 CyTOF FACS Func IP WB Mouse IgG1 Log in to see MEM-148
3.0849469 ABIN3187955 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0849469 ABIN3185341 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0849469 ABIN1539259 WB Rabbit Ig AA 272-300, N-Term Log in to see Polyclonal
3.0849469 ABIN396599 WB Mouse IgG1 kappa AA 600-699 Log in to see 5F12

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope AA 720-769, pThr758
(46), (34), (26), (23), (16), (15), (14), (14), (12), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(478), (212), (117), (43), (38), (21), (21), (13), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(364), (165), (161), (10), (3), (1)
Clonality
Polyclonal
Conjugate This Integrin beta 2 antibody is un-conjugated
(88), (73), (52), (27), (15), (12), (9), (8), (8), (8), (8), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(485), (286), (203), (147), (95), (89), (76), (68), (52), (40), (26), (17), (11), (9), (7), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN1531540) Images
Specificity

CD18/ITGB2 (Phospho-Thr758) Antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at Thr758.

PhosphorylationH:T758 M:T760 R:T757

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758.
Isotype IgG

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Antigen
Alternative Name CD18/ITGB2 (ITGB2 Antibody Abstract)
Background

Synonyms: CD18, cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta, complement receptor C3 beta, integrin beta-2, ITB2

NCBI Gene Symbol: ITGB2

Molecular Weight 84 kDa
Gene ID 3689
OMIM 116920
UniProt P05107
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Application Notes WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Comment

Unigene-Number: Hs.375957 (NCBI Gene Symbol: ITGB2)

Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-Integrin beta 2 antibody (ABIN1531540)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Hattori, Fujiyama, Taylor, Watanabe, Yada, Park, Toyoda, Ishii, Totoki, Choi, Groner, Soeda, Ohki, Takagi, Sakaki, Taudien, Blechschmidt, Polley, Menzel, Delabar, Kumpf, Lehmann, Patterson, Reichwald et al.: "The DNA sequence of human chromosome 21. ..." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000

Kishimoto, OConnor, Lee, Roberts, Springer: "Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family." in: Cell, Vol. 48, Issue 4, pp. 681-90, 1987

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN1531540) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 (ITGB2) (pThr758), (AA 720-769) antibody (ABIN1531540) Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30', usin...
Immunofluorescence (IF) image for anti-Integrin beta 2 (ITGB2) (pThr758), (AA 720-769) antibody (ABIN1531540) Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibod...