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anti-Human Integrin beta 2 antibody for Western Blotting

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Epitope
pThr758
40
31
28
25
16
14
13
9
8
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
464
203
108
39
38
20
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rabbit
360
181
154
5
3
1
Clonality
Polyclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
76
51
19
14
11
9
7
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
489
268
266
152
104
100
82
58
43
34
18
9
7
6
5
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1531540
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13357732 ABIN94005 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
0.13357732 ABIN94010 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 8
0.13357732 ABIN1882257 WB Mouse IgG1 Log in to see 534CT6-2-1 5
0.13357732 ABIN2749019 Func IHC (f) FACS IP IHC (p) WB Mouse IgG1 Log in to see MEM-48 12
0.13357732 ABIN2749020 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 10
0.13357732 ABIN189488 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser745 Log in to see EP1288Y
0.13357732 ABIN2705756 WB Rabbit Center Log in to see Polyclonal
0.13357732 ABIN3044225 IHC (p) WB Rabbit IgG AA 732-748, C-Term Log in to see Polyclonal
0.13357732 ABIN3043260 ICC IHC (p) WB Rabbit IgG AA 404-769 Log in to see Polyclonal
0.13357732 ABIN3043698 IHC (p) WB Rabbit IgG AA 24-58, N-Term Log in to see Polyclonal
0.13357732 ABIN1539259 WB Rabbit Ig AA 272-300, N-Term Log in to see Polyclonal
0.13357732 ABIN396599 WB Mouse IgG1 kappa AA 600-699 Log in to see 5F12
0.13357732 ABIN1873315 IHC WB Rabbit IgG Log in to see Polyclonal
0.13357732 ABIN1539991 WB Mouse IgG1 Log in to see 534CT6-2-1
0.13357732 ABIN1540012 WB Rabbit Ig Log in to see Polyclonal
0.13357732 ABIN2490960 IF ELISA WB Rabbit IgG pThr758 Log in to see Polyclonal
0.13357732 ABIN3023118 IHC WB Rabbit IgG Log in to see Polyclonal
0.13357732 ABIN2488538 WB Mouse IgG1 Log in to see 534CT6-2-1
0.13357732 ABIN1859504 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 124-363 Log in to see Polyclonal
0.13357732 ABIN1859503 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 451-627 Log in to see Polyclonal

Top referenced anti-Integrin beta 2 antibody for Western Blotting

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 6 Antibodies
ELISA 14 Antibodies 20 Antibodies
Flow Cytometry (FACS) 41 Antibodies 126 Antibodies
Functional Studies (Func) 1 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Western Blotting (WB) 30 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope pThr758
(40), (31), (28), (25), (16), (14), (13), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(464), (203), (108), (39), (38), (20), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(360), (181), (154), (5), (3), (1)
Clonality
Polyclonal
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (76), (51), (19), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(489), (268), (266), (152), (104), (100), (82), (58), (43), (34), (18), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) Images
Specificity CD18/ITGB2 (Phospho-Thr758) Antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at Thr758.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758.
Isotype IgG

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) Images back to top
Antigen
Alternative Name CD18/ITGB2 (ITGB2 Antibody Abstract)
Background Molecular weight of target protein: 84 kDa
Synonyms: CD18, cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta, complement receptor C3 beta, integrin beta-2, ITB2
Molecular Weight 84 kDa
Gene ID 3689
OMIM 116920
UniProt P05107
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.375957 (NCBI Gene Symbol: ITGB2)

Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN1531540) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-Integrin beta 2 Antibody (ABIN1531540)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Hattori, Fujiyama, Taylor, Watanabe, Yada, Park, Toyoda, Ishii, Totoki, Choi, Groner, Soeda, Ohki, Takagi, Sakaki, Taudien, Blechschmidt, Polley, Menzel, Delabar, Kumpf, Lehmann, Patterson, Reichwald et al.: "The DNA sequence of human chromosome 21. ..." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000

Kishimoto, OConnor, Lee, Roberts, Springer: "Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family." in: Cell, Vol. 48, Issue 4, pp. 681-90, 1987

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (pThr758) (ABIN1531540) Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30', usin...
Immunofluorescence (IF) image for anti-Integrin beta 2 antibody (ITGB2) (pThr758) (ABIN1531540) Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibod...