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anti-Mouse (Murine) PDK4 antibody for Immunocytochemistry

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
AA 117-387
23
22
13
12
10
6
5
5
5
5
4
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
117
47
12
1
1
1
Host
Rabbit
119
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
9
8
7
7
7
6
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
118
77
55
13
10
4
1
1
1
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Catalog No. ABIN1860175
$ 356.40
Plus shipping costs $45.00

Similar anti-PDK4 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 27 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Western Blotting (WB) 41 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope AA 117-387
(23), (22), (13), (12), (10), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(117), (47), (12), (1), (1), (1)
Host Rabbit
(119), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(9), (8), (7), (7), (7), (6), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(118), (77), (55), (13), (10), (4), (1), (1), (1)
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VDTL VKVRNRHHNV VPTMAQGILE YKDTCTVDPV TNQNLQYFLD RFYMNRISTR MLMNQHILIF SDSKTGNPSH IGSIDPNCDV VAVVQDAFEC AKMLCDQYYL TSPELNLTQV NGKFPGQPIH IVYVPSHLHH MLFELFKNAM RATVEHQENR PSLTPVEATV VLGKEDLTIK ISDRGGGVPL RITDRLFSYT YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL YSMSGYGTDA IIYLKALSSE SVEKLPVFNK SAFKHYQ
Specificity It has been selected for its ability to recognize PDK4 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen PDK4 (AA 117-387)
Isotype IgG

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) (PDK4 Antibody Abstract)
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant PDK4 (Val117~Gln387) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (AA 117-387) (ABIN1860175) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 117-387) antibody