anti-Rabbit PDK4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pdk4
  • LOC100230570
  • Pdk4
  • PDK4
Epitope
N-Term
29
19
18
17
17
12
10
10
8
6
6
6
6
5
5
4
3
2
1
1
1
1
1
1
Reactivity
Bat, Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit
150
73
16
1
1
1
1
1
1
Host
Rabbit
152
20
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
10
8
7
7
7
4
4
4
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
78
60
35
25
17
4
4
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN214801
$ 676.50
Plus shipping costs $45.00

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope N-Term
(29), (19), (18), (17), (17), (12), (10), (10), (8), (6), (6), (6), (6), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit
(150), (73), (16), (1), (1), (1), (1), (1), (1)
Host Rabbit
(152), (20)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(11), (10), (8), (7), (7), (7), (4), (4), (4), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(152), (78), (60), (35), (25), (17), (4), (4), (2), (1)
Supplier Log in to see

Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling Images
Brand IHC-plus™
Specificity Human PDK4
Predicted Reactivity Percent identity by BLAST analysis: Human, Rabbit, Horse, Pig (100%) Mouse, Rat, Bovine, Guinea pig (92%) Dog (91%) Chicken (81%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide from N-Terminus of human PDK4 (Q16654, NP_002603). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Rabbit, Horse, Pig, Platypus (100%), Mouse, Rat, Bovine, Guinea pig (92%), Dog (91%), Lizard (90%), Turkey, Chicken (81%).

Type of Immunogen: Synthetic peptide

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background Name/Gene ID: PDK4
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK4, PDHK4
Gene ID 5166
NCBI Accession NP_002603
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Images back to top
Format Lyophilized
Reconstitution After adding water, will consist of PBS buffer with 2 % sucrose
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody (ABIN214801) Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with PDK4 antibody ...