Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Cross-Reactivity (Details)
Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
Purity
Anti-Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
Endotoxin Level
Low Endotoxin : No
Immunogen
Anti-Phospho Enol Pyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase. Immunogen Type: Native Protein
Anti-Phospho Enol Pyruvate Carboxylase antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. ELISA Dilution: 1:10.000 - 1:40.000 Western Blot Dilution: 1:1.000 - 1:4.000
Restrictions
For Research Use only
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Expiry Date
Expiration date is one (1) year from date of opening.
Anti-Phospho Enol Pyruvate Carboxylase antibody detects PEP. Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate. This reaction is used for carbon fixation in so-called Synonyms: PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody