This product is an IgG fraction antibody purified from monospecific antiserum by a multi- step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified beta-2-Microglobulin [Human Urine]. Cross reactivity against beta-2-Microglobulin from other sources is unknown.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with 0.1 mL of deionized water (or equivalent).
Concentration
5.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer
Preservative
Gentamicin sulfate
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Storage
4 °C
Storage Comment
Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.
Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. Alternate names: B2M, Beta 2 microglobulin, Beta-2 microglobulin