NLRP3 antibody (Pyrin Domain)

Catalog No. ABIN1169101

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: AA 1-93, Pyrin Domain

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes mouse NLRP3/NALP3.

Cross-Reactivity: Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse NLRP3/NALP3 (pyrin domain) (aa 1-93).

Clone: Cryo-1

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-NLRP3 antibody (ABIN1169101)

Dey, Joshi, Oliveira, Pereira, Guimarães-Costa, Serafim, de Castro, Coutinho-Abreu, Bhattacharya, Townsend, Aslan, Perkins, Karmakar, Ismail, Karetnick, Meneses, Duncan, Nakhasi, Valenzuela, Kamhawi: "Gut Microbes Egested during Bites of Infected Sand Flies Augment Severity of Leishmaniasis via Inflammasome-Derived IL-1β." in: Cell host & microbe, Vol. 23, Issue 1, pp. 134-143.e6, (2018) (PubMed).

Li, Ju, Hsieh, Dong, Chen, Hua, Chen: "A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP." in: PLoS ONE, Vol. 12, Issue 7, pp. e0182057, (2017) (PubMed).

He, Wan, Hu, Chen, Wang, Huang, Yang, Zhong, Han: "Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion." in: Cell research, Vol. 25, Issue 12, pp. 1285-98, (2016) (PubMed).

Dashdorj, Jyothi, Lim, Jo, Nguyen, Ha, Yoon, Kim, Park, Murphy, Kim: "Mitochondria-targeted antioxidant MitoQ ameliorates experimental mouse colitis by suppressing NLRP3 inflammasome-mediated inflammatory cytokines." in: BMC medicine, Vol. 11, pp. 178, (2013) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3/NALP3 (NLRP3 Products)

Synonyms: CIAS1 antibody, Nalp3 antibody, avp antibody, cias1 antibody, nalp3 antibody, pypaf1 antibody, agtavprl antibody, AGTAVPRL antibody, AII antibody, AVP antibody, C1orf7 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, MWS antibody, NALP3 antibody, PYPAF1 antibody, AII/AVP antibody, Cias1 antibody, Mmig1 antibody, Pypaf1 antibody, NLR family pyrin domain containing-like antibody, NLR family pyrin domain containing 3 antibody, NLR family, pyrin domain containing 3 L homeolog antibody, NLR family, pyrin domain containing 3 antibody, NLRPL antibody, NLRP3 antibody, nlrp3.L antibody, Nlrp3 antibody

Background: The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

UniProt: Q8R4B8

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome