p53 antibody (AA 181-190)
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- Target See all p53 (TP53) Antibodies
- p53 (TP53) (Tumor Protein P53 (TP53))
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Binding Specificity
- AA 181-190
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This p53 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognises the p53 tumour suppressor protein, binding to both wild type and mutant forms. DO-11 recognises an epitope within the central region of p53 between amino acids 181 - 190.
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
- Clone
- DO-11
- Isotype
- IgG1
- Top Product
- Discover our top product TP53 Primary Antibody
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- Application Notes
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Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/1000, This product does require antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Colon or breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- p53 (TP53) (Tumor Protein P53 (TP53))
- Alternative Name
- p53 (TP53) (TP53 Products)
- Synonyms
- BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody
- Background
- Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
- Gene ID
- 7157
- NCBI Accession
- NP_000537
- UniProt
- P04637
- Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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