Concentration Definition: by UV absorbance at 280 nm
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 312-327 of S. cerevisiae CHK1.
CHEK1
Reactivity: Human, Mouse, Rat
IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.73 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus. Synonyms: CHK1 EC=2.7.11.1 Checkpoint kinase 1