CD11b antibody

Catalog No. ABIN133343

Product Details anti-CD11b Antibody

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Rat CD11b/c (macrophages)

Purification: Purified from ascitic fluid via Protein G Chromatography

Immunogen: Rat Peritoneal Macrophages . Fusion Partner: NSO/U mouse myeloma cell line . Donor: BALB/c spleen

Clone: OX-42

Isotype: IgG2a

Application Details

Application Notes: This clone is reported to work with frozen and paraffin sections (3) in in vivo and in vitro functional tests (4, 5, 6) and cryostat sections (5).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/ml

Buffer: Purified Ig buffered in PBS and 0.02% NaN . (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b/c (ITGAM Products)

Synonyms: CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

Background: Anti-rat CD11b/c monoclonal antibody recognizes most macrophages including resident peritoneal macrophages, kupffer cells, but only approximately 35% of alveolar macrophages (2). ABIN133343also labels dendritic cells extensively, granulocytes and cells with morphology of microglia in the brain. It precipitates three polypeptides of MW 160, 103 and 95 kDa. ABIN133343inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for iC3b called CR3. anti-rat CD11b/c monoclonal antibody should prove useful in the recognition of macrophages and microglia. This clone is reported to work with frozen and paraffin sections (3) in in vivo and in vitro functional tests (4, 5, 6) and cryostat sections (5).

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation