Histone 3 antibody (H3K4me3)

Catalog No. ABIN1387846

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me3

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic metylpeptide derived from human Histone H3 around metylation site of Tri Methyl K4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN1387846)

Hörber, Hildebrand, Lieb, Lorscheid, Hailfinger, Schulze-Osthoff, Essmann: "The Atypical Inhibitor of NF-κB, IκBζ, Controls Macrophage Interleukin-10 Expression." in: The Journal of biological chemistry, Vol. 291, Issue 24, pp. 12851-61, (2016) (PubMed).

Ma, Zheng, Wang, Wang, Wang, Pang, Zhang, Wang, Ding, Li, Wang, Hu, Liu, Zhang, Wang, Wang, Zhao, Liu: "Design, synthesis, and structure-activity relationship of novel LSD1 inhibitors based on pyrimidine-thiourea hybrids as potent, orally active antitumor agents." in: Journal of medicinal chemistry, Vol. 58, Issue 4, pp. 1705-16, (2015) (PubMed).

Zheng, Duan, Ma, Xu, Zi, Lv, Wang, Ye, Zhu, Mobley, Zhu, Wang, Li, Wang, Zhao, Liu: "Triazole-dithiocarbamate based selective lysine specific demethylase 1 (LSD1) inactivators inhibit gastric cancer cell growth, invasion, and migration." in: Journal of medicinal chemistry, Vol. 56, Issue 21, pp. 8543-60, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background:

Synonyms: Histone H3Tri Methyl Lys4, Tri methyl Histone H3Lys4, Tri methyl Histone H3K4, H3 histone family member E pseudogene, H3F3, HIST3H3, Histone H3 3 pseudogene, H31_TETTH, Histone H3, H3S, Histone H3-I/H3-II, Major histone H3, H3F, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l.

Background: Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.

Gene ID: 3020