PCNA antibody (Internal Region)

Catalog No. ABIN1449431

Product Details anti-PCNA Antibody

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Binding Specificity: AA 111-112, Internal Region

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-EAPNQEKVSD YE Remarks: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the the internal region (111-122) of Human PCNA (NP_002583.1) Genename: PCNA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (PCNA Products)

Synonyms: PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody

Background: PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Molecular Weight: 28.8 kDa (NP_002583.1).

Gene ID: 5111

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy