Vimentin antibody (AA 400-500)
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Binding Specificity
- AA 400-500
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
- Specificity
- Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.
- Cross-Reactivity (Details)
- Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.
- Purification
- Immunoaffinity chromatography
- Immunogen
- Synthetic peptide (KLH-coupled) derived from within AA 400-500 of human vimentin
- Isotype
- IgG
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Synonyms
- CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
- Background
- Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).
- Pathways
- Caspase Cascade in Apoptosis
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