CD11b antibody (Integrin alpha M) (APC) Primary Antibody
ITGAM Reactivity: Human, Mouse, Non-Human Primate, Rabbit FACS, Func, IHC (f), IP Host: Rat Monoclonal M1-70
Catalog No. ABIN1690733
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- Human, Mouse, Non-Human Primate, Rabbit
- This CD11b antibody is conjugated to APC
- Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
- The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
- Cross-Reactivity (Details)
- Human, Non-Human Primates, Mouse, Rabbit
- B10 mouse spleen cells enriched for T cells
- Application Notes
- The reagent is designed for Flow Cytometry analysis.
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- For Research Use only
- 0.5 mg/mL
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
Avoid prolonged exposure to light.
- 4 °C
- Storage Comment
- Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
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- Alternative Name
- CD11b (ITGAM Antibody Abstract)
- CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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