MICU1 antibody (AA 113-126)

Catalog No. ABIN1804714

Product Details anti-MICU1 Antibody

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Binding Specificity: AA 113-126

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Horse, Goat, Zebrafish (Danio rerio), Pig, Chicken, Hamster, Monkey, Xenopus laevis, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This MICU1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CBARA1. This antibody is expected to recognize reported isoforms 1 and 2 (NP_006068.2, NP_001182447.1).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EYENRIRAYSTPDK from an internal region of human CBARA1 (NP_006068.2, NP_001182447.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Seq squirt, Ant (100%), Blood fluke, Mosquito, Sea anemone, Water flea, Beetle, Tick (93%), Drosophila (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kD band observed in lysates of cell line K562 (calculated MW of 54.6kD according to NP_006068.2). Recommended concentration: 0.5-2 μg/mL. An additional band of unknown identity was also consistently observed at 37kD. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

References for anti-MICU1 antibody (ABIN1804714)

Niemann, Zehm, Waterstradt, Tiedge, Baltrusch: "Cytosolic and mitochondrial Ca2+ concentrations in primary hepatocytes change with ageing and in consequence of an mtDNA mutation." in: Cell calcium, Vol. 82, pp. 102055, (2019) (PubMed).

Target Details for MICU1

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Alternative Name: MICU1 / CBARA1 (MICU1 Products)

Background: Name/Gene ID: MICU1

Synonyms: MICU1, Ara CALC, CBARA1, CALC, EFHA3, Mitochondrial calcium uptake 1, Allergen Hom s 4, Atopy related autoantigen, Atopy-related autoantigen CALC

Gene ID: 10367

NCBI Accession: NP_006068, NP_001182447

UniProt: Q9BPX6