PAG1 antibody (AA 97-432)
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- Target See all PAG1 Antibodies
- PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
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Binding Specificity
- AA 97-432
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PAG1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kD transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kD molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
- Purification
- Purified
- Immunogen
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Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG.
Type of Immunogen: Recombinant protein - Top Product
- Discover our top product PAG1 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
- Alternative Name
- PAG1 / PAG (PAG1 Products)
- Synonyms
- Cbp antibody, F730007C19Rik antibody, Pag antibody, CBP antibody, PAG antibody, MGC80192 antibody, phosphoprotein associated with glycosphingolipid microdomains 1 antibody, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 antibody, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog antibody, Pag1 antibody, PAG1 antibody, pag1.L antibody, pag1 antibody
- Background
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Name/Gene ID: PAG1
Synonyms: PAG1, Csk-binding protein, CBP, PAG - Gene ID
- 55824
- UniProt
- Q9NWQ8
- Pathways
- p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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