LCP2 antibody (AA 216-434)
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- Target See all LCP2 Antibodies
- LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))
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Binding Specificity
- AA 216-434
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This LCP2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- SLP-76 (SH2 domain-containing leukocyte protein) is an adaptor molecule that is involved in T cell development and activation. Thymocytes from transgenic mice expressing a SLP-76 mutant with deletion of the amino terminus are unable to develop to CD4-CD8- stage. Mature T cells do develop in mice expressing SLP-76 with mutations in proline rich region or SH2 domain, but they fail to upregulate CD69 or proliferate appropriately in response to anti-CD3 stimulation. SLP-76 is cytosolic protein identified as phosphoprotein migrating at 76 kD in SDS-PAGE. Amino-terminal domain of SLP-76 containing several tyrosines is followed by PR (proline rich) region and an SH2 domain. SLP-76 associates with LAT via Gads or Grb 2. The PR region of SLP-76 constitutively associates with carboxy-terminal SH3 domain of Gads and P-1 segment of the PR region was also identified that associates with the SH3 domain of PLC-gamma1. The PR region therefore provides another pathway for PLC-gamma1 recruitment to the LAT signalling complex in T cells. The SH2 domain of SLP-76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
- Purification
- Purified
- Immunogen
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A fusion protein representing amino acids 216-434 of human SLP-76.
Type of Immunogen: Fusion protein - Clone
- SLP-76-03
- Isotype
- IgG2b
- Top Product
- Discover our top product LCP2 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))
- Alternative Name
- LCP2 / SLP-76 (LCP2 Products)
- Synonyms
- AI323664 antibody, BB161688 antibody, SLP-76 antibody, SLP76 antibody, twm antibody, Slp76 antibody, lymphocyte cytosolic protein 2 antibody, Lcp2 antibody, LCP2 antibody
- Background
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Name/Gene ID: LCP2
Synonyms: LCP2, 76 kDa tyrosine phosphoprotein, Lymphocyte cytosolic protein 2, SLP-76, SLP-76 tyrosine phosphoprotein, SLP76 - Gene ID
- 3937
- UniProt
- Q13094
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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