Glutaminase antibody (AA 221-533)
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- Target See all Glutaminase (GLS) Antibodies
- Glutaminase (GLS)
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Binding Specificity
- AA 221-533
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Glutaminase antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Glutaminase (GLS)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against GLS. It has been selected for its ability to recognize GLS in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Glutaminase (GLS) corresdonding to Ile221~Leu533 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product GLS Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Glutaminase (GLS)
- Alternative Name
- Glutaminase (GLS Products)
- Synonyms
- Glut antibody, RATGLUT antibody, gls antibody, si:dz87i4.2 antibody, wu:fa97e05 antibody, GA antibody, PAG antibody, AAD20 antibody, GAC antibody, GAM antibody, GLS1 antibody, KGA antibody, AI314027 antibody, 6330442B14 antibody, B230365M23Rik antibody, CG8772 antibody, CG8872 antibody, Dmel\\CG42708 antibody, Dmel_CG8772 antibody, BA3155 antibody, glutaminase antibody, glutaminase b antibody, Glutaminase antibody, glutaminase kidney isoform, mitochondrial antibody, Gls antibody, GLS antibody, gls antibody, glsb antibody, glsA2 antibody, glsA-2 antibody, Arnit_3113 antibody, Celal_2913 antibody, Celly_0289 antibody, Weevi_2101 antibody, Mesop_4683 antibody, LOC100069617 antibody
- Background
- GLS1, Glutaminase kidney isoform, mitochondrial, K-glutaminase, L-glutamine amidohydrolase
- Pathways
- Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect
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