PCNA antibody (C-Term)

Catalog No. ABIN1922196

Product Details anti-PCNA Antibody

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Binding Specificity: AA 225-261, C-Term

Reactivity: Human, Mouse, Rat, Pig, Dog, Rabbit, Cow, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Specificity: Region between residue 225 and the C-terminus (residue 261) of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:1000 - 1:5000), IHC, IHC-P (1:2000 - 1:10000), IP (2 - 5 μg/mg lysate), PLA (1:1000 - 1:20000), WB (1:5000 - 1:20000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA / Cyclin (PCNA Products)

Synonyms: PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody

Background: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gene ID: 5111

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy