PDK4 antibody (AA 382-410)
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- Target See all PDK4 Antibodies
- PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
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Binding Specificity
- AA 382-410
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PDK4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.
- Purification
- Protein A purified
- Top Product
- Discover our top product PDK4 Primary Antibody
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- Application Notes
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Approved: IF (1:100), IHC, IHC-P (10 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use with mouse in IHC-P. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
- Expiry Date
- 6 months
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- Target
- PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
- Alternative Name
- PDK4 (PDK4 Products)
- Synonyms
- AV005916 antibody, pyruvate dehydrogenase kinase 4 antibody, pyruvate dehydrogenase kinase, isoenzyme 4 antibody, pdk4 antibody, PDK4 antibody, Pdk4 antibody
- Background
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Name/Gene ID: PDK4
Subfamily: PDK/BCKDK
Family: Protein Kinase
Synonyms: PDK4, PDHK4 - Gene ID
- 5166
- UniProt
- Q16654
- Pathways
- PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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