Cyclin D1 antibody

Catalog No. ABIN200701

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes the Cyclin D1 (36kD). Species cross-reactivity: Human and rat.

Purification: Protein A purified

Immunogen: Human cyclin D1.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P, IP, WB (1:2000)

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), ELISA, Immunoprecipitation and Western Blot. Western Blot: 1:2000. Flow Cytometry: Neat, Use 5 μL of the optimal working dilution to stain 1 x10⁶ in 100 μL. Permeabilization is recommended prior to staining. Immunohistochemistry (Paraffin and frozen): 1:1000-1:10000. Note: Paraffin sections require antigen retrieval using sodium citrate buffer,  pH 6.0, heat treatment prior to staining. Positive control: Small intestine, colon.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 / Cyclin D1 (CCND1 Products)

Synonyms: BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody, AI327039 antibody, CycD1 antibody, Cyl-1 antibody, bcl-1 antibody, cD1 antibody, cb161 antibody, cycd1 antibody, etID37810.7 antibody, fb52e01 antibody, fc45c08 antibody, fc83a12 antibody, id:ibd1198 antibody, wu:fb52e01 antibody, wu:fc45c08 antibody, wu:fc83a12 antibody, ccnd1b antibody, prad1 antibody, ccnd1 antibody, ccnd1a antibody, bcl1 antibody, d11s287e antibody, u21b31 antibody, CCND1 antibody, cyclin D1 antibody, cyclin D1 S homeolog antibody, cyclin D1 L homeolog antibody, CCND1 antibody, Ccnd1 antibody, ccnd1 antibody, ccnd1.S antibody, ccnd1.L antibody

Background: Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling