PCNA antibody (Biotin)

Catalog No. ABIN209149

Product Details anti-PCNA Antibody (Biotin)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: Recognizes human PCNA. Reacts with all vertebrate species, insects and S. pombe on Western blots. Species cross-reactivity: mouse, rat, chicken, xenopus.

Purification: Purified

Immunogen: Recombinant PCNA polypeptides.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-P (1:5 - 1:20), WB (1:500 - 1:2000)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 1:500-1:2000. Immunohistochemistry: 1:5-1:20 (Alcohol or formalin-fixed, paraffin-embedded). Positive control: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol and NIH 3T3 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA / Cyclin (PCNA Products)

Synonyms: PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody

Background: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy