AIF antibody (AA 593-606)

Catalog No. ABIN211058

Product Details anti-AIF Antibody

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Binding Specificity: AA 593-606

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Horse, Rabbit, Monkey, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes AIF, Mr 57kD. Another non-specific protein was also detected at Mr 120kD. Species cross-reactivity: Mouse. Predicted for human and rat based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Cat, Horse, Rabbit, Pig (100%) Opossum, Xenopus (92%) Turkey, Chicken, Stickleback, Pufferfish, Zebrafish (83%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 593-606 of human Apoptosis Inducing Factor, AIF (C-KDGEQHEDLNEV-AK), containing a N-terminal cysteine. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Cat, Horse, Rabbit, Pig (100%), Opossum, Xenopus (92%), Turkey, Chicken, Stickleback, Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot Analysis: 1 μg/mL detects AIF from a mouse liver preparation. Mouse liver protein preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with AIF (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 200 μg of protein A purified rabbit IgG in 200 μLof 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol. Liquid at -20 deg C.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1 / AIF / PDCD8 (AIFM1 Products)

Synonyms: AIF antibody, CMTX4 antibody, COWCK antibody, COXPD6 antibody, PDCD8 antibody, CG7263 antibody, DmAIF antibody, Dmel\\CG7263 antibody, GB16024 antibody, DDBDRAFT_0187853 antibody, DDBDRAFT_0191137 antibody, DDB_0187853 antibody, DDB_0191137 antibody, aif antibody, pdcd8 antibody, AIFM1 antibody, PCD8 antibody, AIFsh2 antibody, Hq antibody, Pdcd8 antibody, mAIF antibody, Aif antibody, zgc:91994 antibody, apoptosis inducing factor mitochondria associated 1 antibody, allograft inflammatory factor 1 antibody, Apoptosis inducing factor antibody, apoptosis-inducing factor 1, mitochondrial antibody, apoptosis inducing factor antibody, apoptosis inducing factor, mitochondria associated 1 antibody, apoptosis-inducing factor, mitochondrion-associated, 1 antibody, apoptosis-inducing factor, mitochondrion-associated 1 antibody, AIFM1 antibody, AIF1 antibody, AIF antibody, LOC412212 antibody, aif antibody, aifm1 antibody, Aifm1 antibody

Background: Name/Gene ID: AIFM1
Family: Apoptosis

Synonyms: AIFM1, AIF, COXPD6, PDCD8, Programmed cell death 8

Gene ID: 9131

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect