CDKN1B antibody (pSer10)

Catalog No. ABIN215394

Product Details anti-CDKN1B Antibody

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Binding Specificity: pSer10

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls. Species cross-reactivity: Human, rat and mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF (5 - 10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5-10 μg/IP reaction. Immunofluorescence: 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B / p27 Kip1 (CDKN1B Products)

Synonyms: CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, cdkn1b antibody, sb:cb611 antibody, wu:fb63g10 antibody, wu:fb64g10 antibody, wu:fe18e03 antibody, CDKN1B antibody, Kip1 antibody, p27 antibody, FAM14D antibody, ISG12 antibody, ISG12A antibody, P27 antibody, Cdki1b antibody, AA408329 antibody, AI843786 antibody, p27Kip1 antibody, cdkn1bl antibody, cyclin dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Bb antibody, interferon alpha inducible protein 27 antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Ba antibody, CDKN1B antibody, cdkn1bb antibody, IFI27 antibody, Cdkn1b antibody, cdkn1ba antibody

Background: Name/Gene ID: CDKN1B

Synonyms: CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4

Gene ID: 1027

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy