GADD34 antibody (AA 401-500) (Alexa Fluor 647)

Catalog No. ABIN2174282

Product Details anti-GADD34 Antibody (Alexa Fluor 647)

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Binding Specificity: AA 401-500

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GADD34 antibody is conjugated to Alexa Fluor 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GADD34

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GADD34

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Alternative Name: GADD34 (PPP1R15A Products)

Synonyms: 9630030H21 antibody, Gadd34 antibody, Myd116 antibody, GADD34 antibody, Peg-3 antibody, RGD1624209 antibody, protein phosphatase 1 regulatory subunit 15A antibody, protein phosphatase 1, regulatory (inhibitor) subunit 15A antibody, protein phosphatase 1, regulatory subunit 15A antibody, PPP1R15A antibody, Ppp1r15a antibody

Background:

Synonyms: GADD34, Protein phosphatase 1 regulatory subunit 15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, PPP1R15A

Background: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.

Gene ID: 23645

UniProt: O75807

Pathways: ER-Nucleus Signaling, Unfolded Protein Response