p130 antibody (C-Term)

Catalog No. ABIN218524

Product Details anti-p130 Antibody

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p130 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Purification: Delipidated and defibrinated

Immunogen:

Type of Immunogen: Native protein

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC, IHC-P (1:500), IP (1:100), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Alternative Name: RBL2 / RB2 p130 (RBL2 Products)

Synonyms: RBL2 antibody, RBR2 antibody, RRB2a antibody, PRB2 antibody, RBR-2 antibody, Rb2 antibody, p130 antibody, P130 antibody, retinoblastoma-like 2 (p130) antibody, RB transcriptional corepressor like 2 antibody, related to retinoblastoma 2 antibody, RBL2 antibody, LOC541652 antibody, Rbl2 antibody

Background: Name/Gene ID: RBL2

Synonyms: RBL2, p130, Retinoblastoma-like 2 (p130), RBR-2, Rb2, Retinoblastoma-like protein 2

Gene ID: 5934

UniProt: Q08999

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases