GRIN2B antibody (AA 984-1104)

Catalog No. ABIN220659

Product Details anti-GRIN2B Antibody

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Binding Specificity: AA 984-1104

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: NMDAR2B. By Western blot it recognizes a 180kD band in rat brain membranes. Also recognizes a 70kD band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption with the C-terminal fusion protein that was used to generate the antibody. Species Reactivity: Human, rat, mouse and fish.

Purification: Immunoaffinity purified

Immunogen: C-Terminal Fusion Protein of the NMDAR2B, aa 984-1104, 30kD.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IP, WB (1:200)

Usage: Western blotting 1:200 (NMDAR2B is present in high concentrations in the hippocampus). Immunoprecipitation: 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain using solubilized hippocampal slices. Immunohistochemistry: Not suggested. Note: Above dilutions are with 35S-protein A, with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: GRIN2B / NMDAR2B / NR2B (GRIN2B Products)

Synonyms: NMDAR2B antibody, GluN2B antibody, MRD6 antibody, NR2B antibody, hNR3 antibody, nmdar2b antibody, nr2b antibody, AW490526 antibody, Nmdar2b antibody, glutamate ionotropic receptor NMDA type subunit 2B antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog antibody, glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody, GRIN2B antibody, Grin2b antibody, grin2b.L antibody

Background: Name/Gene ID: GRIN2B
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIN2B, GluN2B, GluR epsilon 2, NMDA receptor 2B, NMDAR2B, NR2B, NR3, MRD6, HNR3

Gene ID: 2904

UniProt: Q13224

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity