SIGIRR antibody can be used for detection of SIGIRR by ELISA at 1:62500. SIGIRR antibody can be used for detection of SIGIRR by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store SIGIRR antibody at -20 °C.
Target
SIGIRR
(Single Immunoglobulin and Toll-Interleukin 1 Receptor (TIR) Domain (SIGIRR))
TIR8 antibody, AI256711 antibody, sc:d148 antibody, RGD1306732 antibody, single Ig and TIR domain containing antibody, single immunoglobulin and toll-interleukin 1 receptor (TIR) domain antibody, SIGIRR antibody, Sigirr antibody, sigirr antibody
Background
SIGIRR acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. It attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through a TIR-TIR domain interaction with TLR4. Through its extracellular domain it interferes with the heterodimerization of Il1R1 and IL1RAP.