Vimentin antibody (truncated)
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Binding Specificity
- truncated
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- ELISA
- Immunogen
- Ni-NTA purified truncated recombinant Vimentin expressed in E. Coli strain BL21 (DE3).
- Clone
- 9E7E7-5G3F10
- Isotype
- IgG1
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Ascitic fluid containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Storage
- -20 °C
- Storage Comment
- Vimentin monoclonal antibody can be stored at -20 °C, stable for one year.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Synonyms
- CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
- Background
- Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
- Gene ID
- 7431
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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