This antibody is not expected to cross-react with other tyrosine protein kinases. Reported variants represent identical protein: NP_005347.3, NP_001036236.1
Purification
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
LCK antibody was raised against a 14 amino acid synthetic peptide near the internal region (near N-Terminus) of LCK (aa 39-52).
ELISA: Antibody detection limit dilution 16000. Western Blot: Approx 60 kDa band observed in Human Thymus lysates (calculated MW of 58.0 kDa according to NP_005347.3). Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
LCK
(Lymphocyte-Specific Protein tyrosine Kinase (LCK))