CDKN1B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CDKN1B antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of CDKN1B.
ELISA: Antibody detection limit dilution 1:32,000. Approximately 26 kDa band observed in human breast and prostate lysates (calculated MW of 22.1 kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27,102 (39) :14028-33, PMID: 16169901). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.