This antibody is expected to recognize both reported isoforms (NP_000094.2 and NP_112503.1), which are identical
Purification
Aromatase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Aromatase antibody was raised against a 12 amino acid synthetic peptide near the internal region of Aromatase.
CYP19A1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 50-55 kDa band observed in human placenta lysates (calculated MW of 57.9 kDa according to NP_000094.2 and NP_112503.1). Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
Aromatase (CYP19A1)
(Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))