This Serotonin Receptor 3A antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
This antibody is expected to recognize both reported isoforms (NP_998786.1 and NP_000860.1).
Purification
Serotonin receptor 3A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Serotonin receptor 3A antibody was raised against a 12 amino acid synthetic peptide near the internal region of Serotonin receptor 3A.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 55 kDa band observed in human colon lysates (calculated MW of 55.3 kDa according to NP_000860.1. Recommended concentration: 0.3-1 μg/mL. A minor band of unknown identity was also consistently observed at 200 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.