This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Purification
GCC185 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GCC185 antibody was raised against a 12 amino acid synthetic peptide near the internal region of GCC185.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 60 kDa band in human lymph node and testis lysates after 0.03 μg/mL antibody staining. Immunohistochemistry: In paraffin embedded human spleen shows select staning of splenocytes. Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
GCC2
(GRIP and Coiled-Coil Domain Containing 2 (GCC2))