Tumor Necrosis Factor antibody (TNF) (Pacific Blue)

Details for Product anti-TNF Antibody No. ABIN2662374, Supplier: Log in to see
Antigen
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Alternatives
anti-Human Tumor Necrosis Factor antibody for ELISA
Reactivity
Human
1257
461
357
144
136
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Mouse
936
522
184
46
10
9
7
7
5
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Pacific Blue
168
100
74
50
49
32
30
28
27
25
25
25
25
25
24
24
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS)
893
854
625
323
286
230
222
190
137
118
97
69
29
29
26
22
20
18
14
9
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Clone MAb11
Isotype IgG1 kappa
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pig (Porcine), Pigtailed Macaque, Sooty mangabey (Cercocebus atys)
Purification The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (Pacific Blue) (ABIN2662374) anti-Tumor Necrosis Factor (TNF) antibody (Pacific Blue)
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (Pacific Blue) (ABIN2662374) anti-Tumor Necrosis Factor (TNF) antibody (Pacific Blue) (Image 2)
Background publications Braun, Björkström, Gupta, Sundström, Ahlm, Klingström, Ljunggren: "NK cell activation in human hantavirus infection explained by virus-induced IL-15/IL15Rα expression." in: PLoS pathogens, Vol. 10, Issue 11, pp. e1004521, 2014 (PubMed).

Iwamoto, Iwai, Tsujiyama, Kurahashi, Takeshita, Naoe, Masunaga, Ogawa, Oguchi, Miyazaki: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007 (PubMed).

Chen, Hou, Jiang, Ma, Meng, Wang, Zhang, Zhang, Tang, Zhang, Wan, Li, Yu, Hu, Yang, He, Wang, Cao: "Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 591-8, 2005 (PubMed).

Enríquez, Klínger, Arturo, Delgado, Tobar, Mosquera: "Peritonitis in continuous ambulatory peritoneal dialysis: cytokines in peritoneal fluid and blood." in: Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, Vol. 18, pp. 177-83, 2002 (PubMed).

Andersson, Wang, Palmblad, Aveberger, Bloom, Erlandsson-Harris, Janson, Kokkola, Zhang, Yang, Tracey: "High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes." in: The Journal of experimental medicine, Vol. 192, Issue 4, pp. 565-70, 2001 (PubMed).

Moormann, Sullivan, Rochford, Chensue, Bock, Nyirenda, Meshnick: "Malaria and pregnancy: placental cytokine expression and its relationship to intrauterine growth retardation." in: The Journal of infectious diseases, Vol. 180, Issue 6, pp. 1987-93, 2000 (PubMed).

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993 (PubMed).

Rathjen, Cowan, Furphy, Aston: "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." in: Molecular immunology, Vol. 28, Issue 1-2, pp. 79-86, 1991 (PubMed).

Danis, Franic, Rathjen, Brooks et al.: "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 ..." in: Clinical and experimental immunology, Vol. 85, Issue 1, pp. 143-50, 1991 (PubMed).

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989 (PubMed).

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988 (PubMed).

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