Histone 3 antibody (H3K9me3)

Catalog No. ABIN2668405

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K9me3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC)

Purification: Protein A Chromatography

Immunogen: This Histone H3 trimethylLys9 antibody was raised against a peptide including trimethyllysine 9 of histone H3.

Isotype: IgG

Application Details

Application Notes: Recommended antibody dilutions for different applications:
ChIP: 10 µg for each ChIP
ICC/IF: 1 µg/mL dilution
WB: 0.5 - 2 µg/mL dilution
CUT&Tag: 1 µg for each 50 µL reaction
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668405)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Maison, Bailly, Quivy, Almouzni: "The methyltransferase Suv39h1 links the SUMO pathway to HP1α marking at pericentric heterochromatin." in: Nature communications, Vol. 7, pp. 12224, (2016) (PubMed).

Quattrocelli, Swinnen, Giacomazzi, Camps, Barthélemy, Ceccarelli, Caluwé, Grosemans, Thorrez, Pelizzo, Muijtjens, Verfaillie, Blot, Janssens, Sampaolesi: "Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle." in: The Journal of clinical investigation, Vol. 125, Issue 12, pp. 4463-82, (2015) (PubMed).

Lu, Venneti, Akalin, Fang, Ward, Dematteo, Intlekofer, Chen, Ye, Hameed, Nafa, Agaram, Cross, Khanin, Mason, Healey, Lowe, Schwartz, Melnick, Thompson: "Induction of sarcomas by mutant IDH2." in: Genes & development, Vol. 27, Issue 18, pp. 1986-98, (2013) (PubMed).

Tennakoon, Wang, Coarfa, Cooney, Gunaratne: "Chromatin changes in dicer-deficient mouse embryonic stem cells in response to retinoic acid induced differentiation." in: PLoS ONE, Vol. 8, Issue 9, pp. e74556, (2013) (PubMed).

Chen, Liu, Lu, Cross, Morris, Shroff, Ward, Bradner, Thompson, Lowe: "Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is susceptible to Brd4 inhibition." in: Genes & development, Vol. 27, Issue 18, pp. 1974-85, (2013) (PubMed).

Rothbart, Krajewski, Nady, Tempel, Xue, Badeaux, Barsyte-Lovejoy, Martinez, Bedford, Fuchs, Arrowsmith, Strahl: "Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation." in: Nature structural & molecular biology, Vol. 19, Issue 11, pp. 1155-60, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020